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transfection
This term is a specialized technical word used almost exclusively within molecular biology and genetics. It describes the specific act of introducing exogenous DNA or RNA into a cell, distinguishing it from "transformation," which traditionally refers to the uptake of DNA by bacteria, or "transduction," which involves a viral vector.
In a laboratory setting, the word carries a neutral, procedural connotation. It is typically paired with descriptors that specify the method of delivery, such as "chemical," "electroporation," or "liposome-mediated," to clarify the mechanism used to bypass the cell membrane.
Meanings
Examples
The transfection efficiency was surprisingly low in the primary cell line.
The transfection efficiency was surprisingly low in the primary cell line.
We need to optimize the transfection protocol before starting the main experiment.
Did you use lipofection or electroporation for the transfection?
I wonder if a different reagent would improve the transfection rate.
Transient transfection allows for rapid protein expression without genomic integration.
Transient transfection allows for rapid protein expression without genomic integration.
The cells showed significant toxicity following the transfection process.
Stable transfection ensures that the transgene is passed to daughter cells.
Check the fluorescence to confirm successful transfection of the GFP plasmid.
Check the fluorescence to confirm successful transfection of the GFP plasmid.