D
Dicread
BerandaKamusRrestriction enzyme

Catatan: Terjemahan untuk entri ini sedang dalam peninjauan kualitas, sehingga sebagian konten untuk sementara hanya ditampilkan dalam bahasa Inggris.

Entri ini belum diterjemahkan ke bahasa Anda, sehingga versi aslinya ditampilkan di bawah.

restriction enzyme

An enzyme that recognizes a specific sequence of nucleotides in a DNA molecule and cuts the DNA at or near that site.
Noun
pl: restriction enzymes

These proteins act as molecular scissors, providing the precision necessary for recombinant DNA technology. They are typically named after the bacteria from which they were isolated, such as EcoRI from Escherichia coli, which dictates the specific nucleotide sequence they target.

In a laboratory setting, the term is used strictly within the context of molecular biology and genetics. It is distinct from general enzymes in that its primary function is the site-specific cleavage of phosphodiester bonds in a DNA strand, rather than catalyzing a general metabolic reaction.

Meanings

Noun

An enzyme that recognizes a specific sequence of nucleotides in a DNA molecule and cuts the DNA at or near that site.

The researchers used a restriction enzyme to cleave the plasmid at a precise location.

Examples

The lab technician added the restriction enzyme to the DNA sample to create sticky ends.

none

The lab technician added the restriction enzyme to the DNA sample to create sticky ends.

Which restriction enzyme should I use to cut this plasmid at the unique site?

School Life

Which restriction enzyme should I use to cut this plasmid at the unique site?

A restriction enzyme acts like molecular scissors for DNA.

Trivia

A restriction enzyme acts like molecular scissors for DNA.

I wonder if this specific restriction enzyme will recognize the sequence in the target gene.

none

I wonder if this specific restriction enzyme will recognize the sequence in the target gene.

The bacteria produce a restriction enzyme to defend themselves against viral infections.

Trivia

The bacteria produce a restriction enzyme to defend themselves against viral infections.

Make sure the restriction enzyme is kept on ice to prevent denaturation.

School Life

Make sure the restriction enzyme is kept on ice to prevent denaturation.

We need a restriction enzyme that produces blunt ends for this cloning strategy.

none

We need a restriction enzyme that produces blunt ends for this cloning strategy.

The reaction failed because the restriction enzyme was contaminated.

School Life

The reaction failed because the restriction enzyme was contaminated.

Each restriction enzyme targets a very specific palindromic sequence of bases.

Trivia

Each restriction enzyme targets a very specific palindromic sequence of bases.

Related Words

DNAenzymenucleotidebase pairpalindromeligasepolymeraseplasmidvectorgenomegenecloningrecombinant DNAbiotechnologymolecular biologycleavagedigestioncutphosphodiester bondadeninethyminecytosineguanineelectrophoresisgelagaroseprimerPCRsequencingmutationbacteriavirusbacteriophagemethylationmethyltransferaseexonucleaseendonucleasecatalystproteinamino acidbufferincubationcentrifugepipettetransformationtransfectionhybridizationprobelocusallelegenotypephenotypechromosomechromatinhistonenucleosomecodontranscriptiontranslationmRNAtRNArRNAoperonpromoteroperatorgenomicsproteomics
Terakhir Diperbarui: May 2026Laporkan Kesalahan