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focus focal lens microscope optics photon laser aperture resolution diffraction refraction magnification imaging fluorescence fluorophore wavelength spectrum prism mirror beam light optical geometry conic ellipse hyperbola parabola axis vertex centroid convergence divergence collimation aberration contrast signal noise detector photodiode sensor scanning voxel pixel slice sectioning depth plane field objective eyepiece diopter focal length caustic interference coherence phase amplitude intensity refractive index dispersion chromaticity luminescence phosphorescence excitation emission filter beam splitter microscopy cytology histology biophysics spectroscopy

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